Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.
Identifieur interne : 000477 ( Main/Exploration ); précédent : 000476; suivant : 000478Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.
Auteurs : Thomas Schwend [Allemagne] ; Inga Redwanz ; Thomas Ruppert ; Andrea Szenthe ; Michael WinkSource :
- Journal of chromatography. A [ 0021-9673 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Protéines végétales.
- composition chimique : Lupinus.
- méthodes : Spectrométrie de masse ESI.
- Milieux de culture, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Plant Proteins.
- chemical : Culture Media.
- chemistry : Lupinus.
- methods : Spectrometry, Mass, Electrospray Ionization.
- Electrophoresis, Polyacrylamide Gel.
Abstract
Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry (ESI-MS-MS) and enzymatic analysis. The proteomic approach revealed the presence of several abundant proteins that had been overlooked using standard enzyme assays, e.g. subtilisin-like protease, glucan 1,3-beta-glucosidase, alpha-amylase, chitinase, thaumatin-like protein, and a secretory pathogenesis-related protein. Several low-abundant proteins were readily detectable by enzymatic assays (peroxidases, phosphatase), but could not be found by ESI-MS-MS. Both data sets support the assumed lytic function of the medium, which appears to be similar to that of the plant vacuole.
DOI: 10.1016/s0021-9673(03)00622-8
PubMed: 13677650
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.</title><author><name sortKey="Schwend, Thomas" sort="Schwend, Thomas" uniqKey="Schwend T" first="Thomas" last="Schwend">Thomas Schwend</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Redwanz, Inga" sort="Redwanz, Inga" uniqKey="Redwanz I" first="Inga" last="Redwanz">Inga Redwanz</name></author><author><name sortKey="Ruppert, Thomas" sort="Ruppert, Thomas" uniqKey="Ruppert T" first="Thomas" last="Ruppert">Thomas Ruppert</name></author><author><name sortKey="Szenthe, Andrea" sort="Szenthe, Andrea" uniqKey="Szenthe A" first="Andrea" last="Szenthe">Andrea Szenthe</name></author><author><name sortKey="Wink, Michael" sort="Wink, Michael" uniqKey="Wink M" first="Michael" last="Wink">Michael Wink</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:13677650</idno><idno type="pmid">13677650</idno><idno type="doi">10.1016/s0021-9673(03)00622-8</idno><idno type="wicri:Area/Main/Corpus">000465</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000465</idno><idno type="wicri:Area/Main/Curation">000465</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000465</idno><idno type="wicri:Area/Main/Exploration">000465</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.</title><author><name sortKey="Schwend, Thomas" sort="Schwend, Thomas" uniqKey="Schwend T" first="Thomas" last="Schwend">Thomas Schwend</name><affiliation wicri:level="3"><nlm:affiliation>Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Redwanz, Inga" sort="Redwanz, Inga" uniqKey="Redwanz I" first="Inga" last="Redwanz">Inga Redwanz</name></author><author><name sortKey="Ruppert, Thomas" sort="Ruppert, Thomas" uniqKey="Ruppert T" first="Thomas" last="Ruppert">Thomas Ruppert</name></author><author><name sortKey="Szenthe, Andrea" sort="Szenthe, Andrea" uniqKey="Szenthe A" first="Andrea" last="Szenthe">Andrea Szenthe</name></author><author><name sortKey="Wink, Michael" sort="Wink, Michael" uniqKey="Wink M" first="Michael" last="Wink">Michael Wink</name></author></analytic><series><title level="j">Journal of chromatography. A</title><idno type="ISSN">0021-9673</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Culture Media (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Lupinus (chemistry)</term><term>Plant Proteins (analysis)</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Lupinus (composition chimique)</term><term>Milieux de culture (MeSH)</term><term>Protéines végétales (analyse)</term><term>Spectrométrie de masse ESI (méthodes)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Lupinus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lupinus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Spectrométrie de masse ESI</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Milieux de culture</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry (ESI-MS-MS) and enzymatic analysis. The proteomic approach revealed the presence of several abundant proteins that had been overlooked using standard enzyme assays, e.g. subtilisin-like protease, glucan 1,3-beta-glucosidase, alpha-amylase, chitinase, thaumatin-like protein, and a secretory pathogenesis-related protein. Several low-abundant proteins were readily detectable by enzymatic assays (peroxidases, phosphatase), but could not be found by ESI-MS-MS. Both data sets support the assumed lytic function of the medium, which appears to be similar to that of the plant vacuole.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">13677650</PMID><DateCompleted><Year>2004</Year><Month>04</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9673</ISSN><JournalIssue CitedMedium="Print"><Volume>1009</Volume><Issue>1-2</Issue><PubDate><Year>2003</Year><Month>Aug</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of chromatography. A</Title><ISOAbbreviation>J Chromatogr A</ISOAbbreviation></Journal><ArticleTitle>Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.</ArticleTitle><Pagination><MedlinePgn>105-10</MedlinePgn></Pagination><Abstract><AbstractText>Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry (ESI-MS-MS) and enzymatic analysis. The proteomic approach revealed the presence of several abundant proteins that had been overlooked using standard enzyme assays, e.g. subtilisin-like protease, glucan 1,3-beta-glucosidase, alpha-amylase, chitinase, thaumatin-like protein, and a secretory pathogenesis-related protein. Several low-abundant proteins were readily detectable by enzymatic assays (peroxidases, phosphatase), but could not be found by ESI-MS-MS. Both data sets support the assumed lytic function of the medium, which appears to be similar to that of the plant vacuole.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schwend</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Redwanz</LastName><ForeName>Inga</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Ruppert</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Szenthe</LastName><ForeName>Andrea</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Wink</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Chromatogr A</MedlineTA><NlmUniqueID>9318488</NlmUniqueID><ISSNLinking>0021-9673</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031297" MajorTopicYN="N">Lupinus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>9</Month><Day>19</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>4</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>9</Month><Day>19</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">13677650</ArticleId><ArticleId IdType="pii">S0021-9673(03)00622-8</ArticleId><ArticleId IdType="doi">10.1016/s0021-9673(03)00622-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li></region><settlement><li>Heidelberg</li></settlement></list><tree><noCountry><name sortKey="Redwanz, Inga" sort="Redwanz, Inga" uniqKey="Redwanz I" first="Inga" last="Redwanz">Inga Redwanz</name><name sortKey="Ruppert, Thomas" sort="Ruppert, Thomas" uniqKey="Ruppert T" first="Thomas" last="Ruppert">Thomas Ruppert</name><name sortKey="Szenthe, Andrea" sort="Szenthe, Andrea" uniqKey="Szenthe A" first="Andrea" last="Szenthe">Andrea Szenthe</name><name sortKey="Wink, Michael" sort="Wink, Michael" uniqKey="Wink M" first="Michael" last="Wink">Michael Wink</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Schwend, Thomas" sort="Schwend, Thomas" uniqKey="Schwend T" first="Thomas" last="Schwend">Thomas Schwend</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/ThaumatinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000477 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000477 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= ThaumatinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:13677650
|texte= Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:13677650" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ThaumatinV1
| This area was generated with Dilib version V0.6.37. |  |